FastQCFastQC Report
Mon 8 Mar 2021
TC8_B7_mArs_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B7_mArs_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32494178
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2968740.9136221264006125TruSeq Adapter, Index 3 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC2680450.8249016177605724TruSeq Adapter, Index 3 (100% over 50bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1565100.48165551379696386No Hit
GTCCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCT793960.24433915515573282No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG647070.19913413412088773No Hit
CCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCT570980.17571763163234966No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT549130.16899335013182976No Hit
GCCGCCTTTCCACCAGGGATCCACTACAGCACTTGGTAACATGCTCACTC474310.14596768688840198No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT469760.14456743604962097No Hit
CTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCTT422650.13006945428808817No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTTCCATAGAACTAGCTTCTTCT382250.11763645782946101No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG378520.11648855988909768No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT370150.11391271384061476No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT328880.1012119771117152No Hit
ATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTG325670.10022410783864111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)