FastQCFastQC Report
Mon 8 Mar 2021
TC8_B7_mArs_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B7_mArs_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32494178
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2999930.9232207689635971Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2734810.841630768441042Illumina Single End PCR Primer 1 (100% over 50bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT733730.22580352701951717No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC520830.16028409766204887No Hit
CTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAAGGC513500.15802830894814449No Hit
GTTGTGGTCAGCTGTGGAAAGGAAGCCTCGTTGCCACCATGAACTTCTCC485660.14946062029942717No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC480090.1477464670748095No Hit
CTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGA413010.12710276899449496No Hit
CGGAGAGCTCCGGGGAGGTCACCCACACCCTTCAGGATGAAAGCTGTGGT401220.12347442671114808No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA394840.12151099806248368No Hit
GCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAG382010.11756259844455828No Hit
GGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAA370900.11414352441843581No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG353120.10867177498689151No Hit
GGCACCATCACCAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTAT343400.10568046989833071No Hit
CTTAGATCCAGGGTGCCCCTCCAACCAGGATGAGGCTCTTCATCGCTCTT341910.10522192621705956No Hit
GGAAGCCTCGTTGCCACCATGAACTTCTCCGGCAAGTACCAATTGCAGAG326850.10058724981441292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)