FastQCFastQC Report
Sat 13 Feb 2021
TC8_C1_fCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_C1_fCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15408054
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCATTAATCTTTTCTACATTAAAGTTCCTTTCCATAGAACTAGCTTCT688320.4467274063291834No Hit
GTCCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCT527890.3426065355170744No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTTCCATAGAACTAGCTTCTT448230.2909063013408442No Hit
CCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCT441530.28655792613395564No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTTCCATAGAACTAGCTTCTTCT324890.2108572568606003No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT262400.1703005454160532No Hit
GGGCGATCTCACTCTTGTGTGCTTCTCGGCGAAACACACCCCTGGAAAAA243540.15806019371427438No Hit
CGGGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTC230550.14962953790270986No Hit
GGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAG205140.13313816267777878No Hit
GGTCTGTTGAGGGTTTGGAGGAGGTGTTGGAAGGACTTGTGGATGTCAGC198980.12914025353234093No Hit
GTTGGGTTTTCCTTAAAGGAGGTGCACATGGCCTCAGCCTCTGGCCTTTC188950.12263067094650627No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT176830.11476465490061237No Hit
CTGGGGACTGATCCTTCTGGGAGGTGTCTGTCTCCTGAACATCCTCAGCC176540.11457644164538884No Hit
GGGGCATAGAAATAAGGATGTCTTCTGGCAACTTCATGCAAATAGTGTCC171750.11146767787807597No Hit
GGGCAGTTACGATAACATTGTAAATTTGGTCATCTCCTAAAAGTGCACCT169710.11014369497926214No Hit
GCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCTTCCTGGACCTG162040.10516577888421211No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTT161350.10471796113902508No Hit
CTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCTT158660.10297212094402058No Hit
GTCTGTTGAGGGTTTGGAGGAGGTGTTGGAAGGACTTGTGGATGTCAGCC156580.1016221775962104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)