FastQCFastQC Report
Sat 13 Feb 2021
TC8_C1_fCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_C1_fCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15408054
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC468200.3038670555022717No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC378860.24588439266892498No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT373720.24254847497289403No Hit
CTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATT327780.2127328993005866No Hit
GGCACCATCACCAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTAT285640.1853835662829323No Hit
CCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGGGT255310.16569905583145023No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC247990.16094829366511826No Hit
CGGAGAGCTCCGGGGAGGTCACCCACACCCTTCAGGATGAAAGCTGTGGT237450.15410771535458015No Hit
CTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAAGGC232200.15070040642380927No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC228720.14844184736112684No Hit
AGGCACCATCACCAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTA225670.14646236312515518No Hit
CATCACCAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTATCTACC210320.13650004082280603No Hit
CAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTATCTACCAAAATG205880.13361843098421125No Hit
ACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATT201770.13095099484983633No Hit
GTTGTGGTCAGCTGTGGAAAGGAAGCCTCGTTGCCACCATGAACTTCTCC188220.12215689275232292No Hit
CATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATC184210.11955435774043888No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA173980.11291497290962246No Hit
CCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAAT164390.10669095526274766No Hit
GTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTA162660.10556816584365553No Hit
CGTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACT160480.10415332137335448No Hit
CACCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGG155630.1010056169325471No Hit
CACCATCACCAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTATCT155470.10090177513656169No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)