FastQCFastQC Report
Mon 8 Mar 2021
TC8_C2_fCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_C2_fCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14459407
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCT319860.22121239135187218No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG278550.19264275498988306No Hit
CCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCT259040.17914980884070833No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG245520.16979949454358673No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG238190.16473013035735146No Hit
CGGGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTC223840.15480579528607225No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC220780.15268952592592488No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA204820.14165172887103875No Hit
GGCCGCCTTTCCACCAGGGATCCACTACAGCACTTGGTAACATGCTCACT183930.12720438673591525No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC164780.11396041345264021No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC164270.11360770189261565No Hit
GTGGTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGT157460.10889796517934656No Hit
CTCCCCTTTAAAGAACAGCATGGTCCCATTGTGATCCATGGTCGCAGCAT156470.10821328979812243No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC150270.10392542377429448No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG149260.10322691656718702No Hit

[FAIL]Adapter Content

Adapter graph