FastQCFastQC Report
Mon 8 Mar 2021
TC8_C3_fCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_C3_fCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20244073
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG518600.2561737452734931No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC412130.2035805739289717TruSeq Adapter, Index 12 (100% over 50bp)
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG293340.14490167072604412No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG289560.14303445754221494No Hit
GTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTA277170.13691414766188603No Hit
GTGGGAAGACTGGGCCTGGCACATCTCACTTAGCCGCAGGAGCCGGTTTC263170.12999854327733357No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC257870.1273804930460387No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA239460.11828647328035223No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG238430.11777768238634588No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC236090.11662178851064212No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG235600.11637974235718276No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC230720.11396916025742448No Hit
GGGAAGACTGGGCCTGGCACATCTCACTTAGCCGCAGGAGCCGGTTTCTC202990.10027132385859308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)