FastQCFastQC Report
Mon 8 Mar 2021
TC8_D1_fArs_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_D1_fArs_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11037852
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT952910.8633110862512018No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT638710.5786542526571293Illumina Single End PCR Primer 1 (100% over 50bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG316890.28709390196570855No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC265410.24045439275685163No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG176780.16015797276499089Illumina Single End PCR Primer 1 (100% over 50bp)
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATG170440.15441410158425753No Hit
GGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG158470.14356960031716315No Hit
CGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCAT150370.1362312160010843No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA144650.13104904831121128No Hit
AGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCG127580.11558408284510428No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC119170.10796484678359522No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG116910.10591734696207197No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC116400.10545530054217071No Hit
GGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGC113750.10305447110542884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)