FastQCFastQC Report
Mon 8 Mar 2021
TC8_D3_fArs_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_D3_fArs_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26514783
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC756780.2854181382514049No Hit
GTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACT713860.26923094184855295No Hit
GGCAGAAGGTAGGGAGAGGGGCTCTAGCGGTGGCCAGTGGAGCAGCTGGG553850.20888347455078174No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT528780.1994283717124896No Hit
GCCAATGCGGGGGTGGGGAGTGGAAGCGGGCACCTCACTGGGCAGTCAGA443410.1672312385132475No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT430850.16249425839163006No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGC388450.14650317900018264No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC370990.13991817319417624No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG329560.12429292745861809No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG328320.12382526381603802No Hit
GCCAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTC325250.12266741915255351No Hit
ATTTTTTATTAATTGCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAA315880.11913354146628316No Hit
GGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTC314990.11879787965830232No Hit
GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT299080.11279745340552098No Hit
GTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGA290150.10942952088274681No Hit
GGCTGAGTAAGCATTAGACTGTAAATCTAAACACAGAGGTTTAAATCCTC286050.10788321367744176No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)