FastQCFastQC Report
Mon 8 Mar 2021
TC8_D3_fArs_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_D3_fArs_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26514783
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT995050.3752812157655599No Hit
CTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAG796520.3004060036998983No Hit
AAGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGC682260.25731306192473835No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT417840.15758756162552792No Hit
CCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCA409230.15434031649438731No Hit
ACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAA326060.12297290911262597No Hit
AGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCT288130.10866768172305993No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG280710.10586924282955663No Hit
CCCACCTGAAGACACTTGGCGAGAAAGCCAGACCTGCGCTGGAGGACCTG275840.10403253158813332No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA272600.10281057174784346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)