FastQCFastQC Report
Mon 8 Mar 2021
TC8_D5_fArs_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_D5_fArs_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32360943
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3247721.0035925096496725TruSeq Adapter, Index 5 (100% over 50bp)
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT573210.17713019055099846No Hit
ATTTTTTATTAATTGCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAA457050.14123506845891357No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG419670.12968410716585113No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGC361160.11160366989305597No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC360490.11139663019090636No Hit
CACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGGTG333740.10313049282896361No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA324410.1002473877229103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)