FastQCFastQC Report
Mon 8 Mar 2021
TC8_D6_fArs_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_D6_fArs_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26463182
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG816960.30871570924464037Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT686180.2592961043006846Illumina Single End PCR Primer 1 (100% over 50bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC644710.24362527529758135No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA577310.21815592697809355No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT500820.1892516175870309No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT488790.18470567900715795No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCTCGGGTCTTCCCGGAGT365610.13815798871050353No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG361770.13670691604660393No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC353070.13341932954245636No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCTCGGGTCTTCCCGGA326620.12342431080283542No Hit
CTTAGATCCAGGGTGCCCCTCCAACCAGGATGAGGCTCTTCATCGCTCTT315250.1191277753370702No Hit
CGGAGAGCTCCGGGGAGGTCACCCACACCCTTCAGGATGAAAGCTGTGGT313820.11858740192317008No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT303060.11452137539620141No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCC283120.10698637828209774No Hit
CTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAAGGC278930.10540304639101979No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)