FastQCFastQC Report
Fri 18 Dec 2020
BPA10MG_F_6_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBPA10MG_F_6_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58203473
Sequences flagged as poor quality0
Sequence length35-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCGGCTCTGCTTTTTCCA1162560.19974065808753372No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCGGCTCTGCTTTTTCCAGGGGT958780.16472900165253024No Hit
CTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAG747180.12837378278096911No Hit
CAACCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCGGCTCTGCTTTTT737110.12664364547455786No Hit
CACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCGGCTCTGCTTTTTCCAGGG602360.10349210604666151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)