FastQCFastQC Report
Tue 5 Jan 2021
G189_M12_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG189_M12_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46455002
Sequences flagged as poor quality0
Sequence length35-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCGGCTCTGCTTTTTCCA748890.1612076133373108No Hit
GTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCTGAGCGGGAATAGTGG697720.15019265309686136No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCGGCTCTGCTTTTTCCAGGGGT573130.12337315150691415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)