FastQCFastQC Report
Tue 5 Jan 2021
G189_M28_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG189_M28_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47478657
Sequences flagged as poor quality0
Sequence length44-75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAATAAGTA1520370.32022177880894986No Hit
GCGGTCTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAATAAG1152630.2427680294326775No Hit
GGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTG1047830.22069495352406449No Hit
GCGGAATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAAATTAGGATAGATAAGATTAA1021150.21507558648931457No Hit
GTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAAT936890.19732866496202703No Hit
GTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGTTGGGTTGTTTGATCCTGTTTCGTGGAGGAAGAGGAG932110.19632189680512654No Hit
GTGAATGATAAAATTATGAGGTTTAGGGTTCCTACATGGTTTTGGATTAAGATGAGGGCAATTAGCAGTGGAATA902230.19002854271973194No Hit
CTAGAATAATGGAGATGCGAATTATTCCGTAACTACCTAATTTTAGAAGAATAGCTGCTAGAATTATTGACCCAG867960.18281056264923415No Hit
GTCTAGAATAATGGAGATGCGAATTATTCCGTAACTACCTAATTTTAGAAGAATAGCTGCTAGAATTATTGACC829970.17480907263236195No Hit
CTCTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGTTTGGAATGGCACACAACTTATCTC767850.16172529901172225No Hit
AGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTAT702540.14796964454997116No Hit
GTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATT640450.13489218955793125No Hit
GTTGCGGTCTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAAT613230.12915908720838504No Hit
GTTGGGATTAAGGTTGCTTCAAATAAAATATAAAATATAATTAGTTCAGTTGCTGAAAAGGTTATGATTAGGAGA592800.12485610113192544No Hit
GGGGTGTTTAGTGGATTAGCTGGTATGTAGTTGTCTGGGTCTCCTAGTATGTCTGGGAAAAATAATACTAGGGTT583340.12286362691345713No Hit
GGCAGTTACGATAACATTGTAAATTTGGTCATCTCCTAAAAGTGCACCTGGTTGACCTAATTCTGCTCGAATTAA579920.12214330325308065No Hit
TGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGT556520.11721477294524148No Hit
GTGGTATTGGTAGGGGAACTCATAGACTTAATGCTAGTGTGAGTGATAGGGTAGGTGCAATAATAAATAAGGATA556410.11719160464037556No Hit
GGCGAGGCCTAGTTTTATGGATAGGGCTATTAATGTTATGTTAAGGATAAGACCGTTTGTTTGTTGTTGAAATAT555940.11709261279231215No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAA553440.11656606040899599No Hit
GTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAATAAGTACGGAT537710.11325299281317078No Hit
GTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTAT520090.10954185161555854No Hit
GCTTGATAGTCAGGTTAGTGGTAGTAGCATTAGTGAGGGAAGAATAATTTTTAGCATTGTAGTAGGTTGAGATTT511190.10766732513095305No Hit
GGGGCATAGAAATAAGGATGTCTTCTGGCAACTTCATGCAAATAGTGTCCCATAAAGGTGGTTGGGTTTTCCTTA499640.10523465312003244No Hit
CAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGG499230.1051482985291686No Hit
GTTTAGTGGATTAGCTGGTATGTAGTTGTCTGGGTCTCCTAGTATGTCTGGGAAAAATAATACTAGGGTTATGAG498880.10507458119550432No Hit
CGCGATAATAAATGGTAAGATGAAGTGGAAAGCGAAGAATCGGGTCAAGGTGGCTTTGTCTACTGAGAAGCCCC485720.10230280944972811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)