FastQCFastQC Report
Tue 5 Jan 2021
G189_M30_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG189_M30_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46561983
Sequences flagged as poor quality0
Sequence length61-75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAATAAGTA1743320.3744084524922403No Hit
GCGGTCTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAATAAG1294130.27793704576542627No Hit
GGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTG1220620.2621494879202202No Hit
GTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGTTGGGTTGTTTGATCCTGTTTCGTGGAGGAAGAGGAG1153000.24762691056349553No Hit
GTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAAT1109670.2383210354249732No Hit
GTGAATGATAAAATTATGAGGTTTAGGGTTCCTACATGGTTTTGGATTAAGATGAGGGCAATTAGCAGTGGAATA1090170.2341330694614102No Hit
CTAGAATAATGGAGATGCGAATTATTCCGTAACTACCTAATTTTAGAAGAATAGCTGCTAGAATTATTGACCCAG993130.21329203268683808No Hit
CTCTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGTTTGGAATGGCACACAACTTATCTC981090.21070623216369455No Hit
GCGGAATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAAATTAGGATAGATAAGATTAA978770.2102079715977732No Hit
GTCTAGAATAATGGAGATGCGAATTATTCCGTAACTACCTAATTTTAGAAGAATAGCTGCTAGAATTATTGACC898260.19291704135539073No Hit
AGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTAT826690.17754613243168788No Hit
GTGGTATTGGTAGGGGAACTCATAGACTTAATGCTAGTGTGAGTGATAGGGTAGGTGCAATAATAAATAAGGATA802560.17236379301113527No Hit
GTTGCGGTCTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAAT774700.16638037087037294No Hit
GTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATT746720.1603711766313733No Hit
GTTGGGATTAAGGTTGCTTCAAATAAAATATAAAATATAATTAGTTCAGTTGCTGAAAAGGTTATGATTAGGAGA742290.1594197566714459No Hit
GGCAGTTACGATAACATTGTAAATTTGGTCATCTCCTAAAAGTGCACCTGGTTGACCTAATTCTGCTCGAATTAA711630.15283498557181296No Hit
TGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGT668720.1436193127771212No Hit
GGGGTGTTTAGTGGATTAGCTGGTATGTAGTTGTCTGGGTCTCCTAGTATGTCTGGGAAAAATAATACTAGGGTT656370.14096693433353127No Hit
GTGCAATAATAAATAAGGATATAGAGGTTGTTAAAGGGCGTATTGGTTCTTTTATAAATAATTTTATGGCGT652880.14021739580979617No Hit
GGCGAGGCCTAGTTTTATGGATAGGGCTATTAATGTTATGTTAAGGATAAGACCGTTTGTTTGTTGTTGAAATAT640850.13763374296150574No Hit
GGCGTATTGGTTCTTTTATAAATAATTTTATGGCGTCTGCAAATGGTTGTAAAATGCCGTATGGACCAACAATGT635050.1363880915467024No Hit
GTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAATAAGTACGGAT629470.13518968897866743No Hit
GGGGCATAGAAATAAGGATGTCTTCTGGCAACTTCATGCAAATAGTGTCCCATAAAGGTGGTTGGGTTTTCCTTA625540.13434565276139548No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAA608220.1306258799158103No Hit
GCTTGATAGTCAGGTTAGTGGTAGTAGCATTAGTGAGGGAAGAATAATTTTTAGCATTGTAGTAGGTTGAGATTT597990.12842880854107955No Hit
GTTTAGTGGATTAGCTGGTATGTAGTTGTCTGGGTCTCCTAGTATGTCTGGGAAAAATAATACTAGGGTTATGAG591060.12694046986787483No Hit
GTGGGCTCATACAATAAAGCCTAGAAAGCCAATAGACATTATTGCTCATACTATTCCTATATAGCCGAAAGGTT590460.12681160937668828No Hit
GTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTAT585180.1256776370542466No Hit
GTAAATTTGAATTAAAATTGATAGGGGAGCAATTTTTTGTCATGTAAGAAGAATAAGTCCTATGTGCAGTGGGAT570780.12258498526576929No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGG563440.12100859192358711No Hit
CTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGTTTGGAATGGCACACAACTTATCTCCAA556200.11945367532993602No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCGAGAAGCAGGTGT540420.11606464441172963No Hit
CTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACGGCTGTAATAAGTACGG525650.11289252865368728No Hit
GGCTCATACAATAAAGCCTAGAAAGCCAATAGACATTATTGCTCATACTATTCCTATATAGCCGAAAGGTTCTTT517540.11115076434781569No Hit
GGGGTATGCTATATATTTTGTTAGTGGGTCTAGAATAATGGAGATGCGAATTATTCCGTAACTACCTAATTTTAG516670.11096391663559517No Hit
CAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGG507080.1089042964514634No Hit
GCTAGGGCCGCGATAATAAATGGTAAGATGAAGTGGAAAGCGAAGAATCGGGTCAAGGTGGCTTTGTCTACTGAG471580.1012800507229256No Hit
CGCGATAATAAATGGTAAGATGAAGTGGAAAGCGAAGAATCGGGTCAAGGTGGCTTTGTCTACTGAGAAGCCCC467230.10034581216182309No Hit
GTCTGAGGAGAATATATTTGAAAAGTTTTTATAATTTTCGTCGGTTTGTCATAGAAGTGTTAGGCTGGTTAAA466930.1002813819162298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)