FastQCFastQC Report
Tue 5 Jan 2021
G189_M4_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG189_M4_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34144572
Sequences flagged as poor quality0
Sequence length38-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1124920.32945792965277176TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT990740.2901603218221625TruSeq Adapter, Index 14 (97% over 44bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA374030.10954303366286156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)