FastQCFastQC Report
Sat 6 Mar 2021
TC2_B1_mBPA_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC2_B1_mBPA_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36059933
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTG1217550.33764621803373845No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGG538690.14938741012081191No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG489160.1356519436683368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG488050.13534412279690036No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426790.11835573848681305No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTG416650.11554375322882601No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTG372110.10319209411731296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)