FastQCFastQC Report
Sat 6 Mar 2021
TC2_B4_mBPA_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC2_B4_mBPA_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28810104
Sequences flagged as poor quality0
Sequence length35-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTG1357960.4713485241150119No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG635570.22060663161785182No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTG563230.19549738522290652No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG299870.10408501128631815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)