FastQCFastQC Report
Sat 6 Mar 2021
TC2_E4_fBPA_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC2_E4_fBPA_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47478657
Sequences flagged as poor quality0
Sequence length35-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAACACACCCATTATTTAAAATTATTAACCACTCATTCATTGACCTACCTGCCCCATCCAACATTTCATCAT904050.19041187285478609No Hit
GTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCTGAGCGGGAATAGTGG798250.16812817599284666No Hit
CATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCA703790.14823292074162925No Hit
CATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACAT667250.14053683110708037No Hit
CTTGCCATCATCTACTTCACAATCTTCTTAGGTCCTGTAATCACAATATCCAGCACCAACCTAATACTAATATGA560360.1180235574060151No Hit
CGAAAAACACACCCATTATTTAAAATTATTAACCACTCATTCATTGACCTACCTGCCCCATCCAACATTTCATCA530030.11163542389162355No Hit
CAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTCCTAATT489050.10300417722430523No Hit
ATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATT482030.10152561813195349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)