FastQCFastQC Report
Sat 6 Mar 2021
TC2_F6_fBPAhi_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC2_F6_fBPAhi_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58203473
Sequences flagged as poor quality0
Sequence length48-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCAA1783070.30635113475101394No Hit
ATTTTTTATTAATTGCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGGTGTTGATTT1253610.21538405448760764No Hit
CACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGAT717230.12322804173558509No Hit
GTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGC714090.1226885550283228No Hit
CTCGGAGAATCTTTATTAAGCAAGGGCCACCAGAGGGCCAGAGAGGTGCTTGAGACAGGGCCACCTGGCTGGATA643530.11056556710971525No Hit
CCATTTTTTATTAATTGCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGGTGTTGAT608620.10456764324012074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)