FastQCFastQC Report
Tue 4 May 2021
TC5_B3_mDEHP_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_B3_mDEHP_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60083008
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG3363630.5598304931737106TruSeq Adapter, Index 3 (97% over 36bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC2169340.3610571561264043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1451370.24156080867322755No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGC1435990.2390010167267258No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC1291300.21491933293353088No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC1174860.19553947765065294No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA939470.15636201170221037No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTATG925500.15403689509020588TruSeq Adapter, Index 3 (97% over 36bp)
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC898000.14945989388547257No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG865900.144117285206493No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATGAGAGTAGTGGTATTTCACC859920.14312199548997281No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAA853780.14210007594826146No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA841930.14012780452004No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC795810.13245175740868367No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTT766200.12752357538424175No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC756300.12587585495053777No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG753710.1254447846552556No Hit
GGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTG746880.1243080239924073No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC736060.12250718206385405No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG724190.12053158190748373No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTA708940.11799342669394981No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC694970.1156683100819453No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCAC687010.11434347627868433No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT669540.11143583224062284No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACG662880.11032736576704015No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGC660170.10987632310286463No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGG653200.10871626134297405No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG652860.1086596729644428No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGCC650110.10820197284396947No Hit
GTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCG647070.10769600616533713No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCC635620.10579030930009364No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAG622700.1036399509159062No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAG607810.10116171280905244No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAG601200.100061568155842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)