FastQCFastQC Report
Tue 4 May 2021
TC5_C7_mPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_C7_mPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60432455
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2182600.36116355028105346No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1576700.26090285426928295No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1433330.2371788470284717No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1363590.2256386903361778No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1257450.20807528007922232No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1152490.19070712914112126No Hit
CCCCCCCCCCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1143240.1891764946501015No Hit
CCCCCCCCCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1038820.1718976996714762No Hit
CCCCCTCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT956430.15826429689146337No Hit
CCCCTCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT887880.14692105425801416No Hit
CCCCCCCCCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT831750.1376329986925072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA814400.1347620248093512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG799860.13235603286346717No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT789910.13070956657312696No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT786020.1300658727169035No Hit
CCCCCCCCCCGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT748480.1238539787933487No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT667890.11051842921158837No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT659640.10915326871959777No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCG634190.10494195544430555No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT618930.1024168222191205No Hit
CCCCCCCCCCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT618790.10239365585925642No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA615230.10180456842271259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)