FastQCFastQC Report
Tue 4 May 2021
TC5_D4_fCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_D4_fCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69942387
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG17597912.5160579663945413TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTATG3926680.56141635543551TruSeq Adapter, Index 7 (97% over 36bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC2146560.3069040237359929No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC1372020.19616430877602162No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGC1369160.19575540079866022No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC1289580.18437746484116993No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA966530.13818945012557265No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC949080.13569453956439892No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA900080.12868877351869618No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATGAGAGTAGTGGTATTTCACC897260.1282855845340251No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG894760.12792814749087703No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAA869230.12427800040624865No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG819160.11711925130607852No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC816450.11673178955130599No Hit
GGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTG794010.11352343465200866No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCAC772780.110488079281595No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC763070.1090997938060078No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC737410.10543105999513572No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG708500.1012976580281711No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTA701990.10036689196781345No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG700670.10017816520903125No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTT700150.10010381830405646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)