FastQCFastQC Report
Tue 4 May 2021
TC5_E4_fPb_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_E4_fPb_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55420704
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG8678361.5659057669133905TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTATG2316890.41805495650145474TruSeq Adapter, Index 19 (97% over 38bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC1606350.289846552652958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1063640.1919210553514441No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGC1045480.18864430159530274No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC975480.1760136428436564No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC828640.14951812954234575No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA731400.13197234015648737No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC703190.1268821846795739No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA671460.12115688750543478No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG633970.11439226755401737No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAA605710.10929309017799559No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATGAGAGTAGTGGTATTTCACC605570.10926782886049229No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG591640.10675432776891466No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG555230.10018458083823692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)