FastQCFastQC Report
Tue 4 May 2021
TC5_E7_fPb_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_E7_fPb_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57636273
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17143722.9744671380816037No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG10052331.7440978531002516TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTATG1741040.30207366114738193TruSeq Adapter, Index 7 (97% over 36bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC1271880.22067353314118698No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC972350.16870452397225616No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC935680.16234221112805125No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA848350.1471902945563465No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810380.1406024293069748No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC803650.1394347618556113No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGC717890.12455524318860799No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA690350.11977700223607449No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG656900.11397336534928273No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG626280.10866073869835408No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAA578340.10034306000320319No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA578270.1003309148736942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)