FastQCFastQC Report
Tue 4 May 2021
TC4_A1_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_A1_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42742154
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1608370.3762959629970918No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT1440400.3369975223990817No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG1403760.328425188866242No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT1190440.2785166138327984No Hit
GTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAAATCTCATTCAGGG773080.18087062247728555No Hit
CTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCATG753780.17635517386419036No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT750010.1754731406376946No Hit
CACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGGTGTT637530.14915719970500316No Hit
GTCTTCAGTTGCTCCGCTGTAGCCTTGGGCTTGTGCTTCACCAGCTCAGCAA536650.12555520716152957No Hit
CCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCAGCCTTGCAACATGTATCCAG523990.12259326004019358No Hit
CCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGCTGTAGC509860.11928739014884462No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG503980.11791169906879284No Hit
GTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCC499270.11680974243834319No Hit
GTCCTTGTCAGCAGCCTTGCAACATGTATCCAGGAACTGTGCAAAGTCATCC480250.1123598029242981No Hit
ATTTTTTATTAATTGCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATT476360.11144969436963799No Hit
GTGGTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTT470540.11008804095366836No Hit
CTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTGT470160.10999913574781467No Hit
GTGTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTC459090.10740918672465594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)