FastQCFastQC Report
Tue 4 May 2021
TC4_A3_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_A3_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39003423
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA1301130.3335937976520676No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC1132060.2902463201755395No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1032050.26460498095256924No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT974630.24988319614922005No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG874970.22433159263995883No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT763140.19565975017115808No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC634550.16269084895446229No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATAT619050.15871683877591974No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTA572340.14674096681206672No Hit
GGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCT554550.1421798286781137No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG551090.14129272705116164No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATG508630.13040650303948964No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA499150.12797594713674232No Hit
GGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGG477590.12244822717226639No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGG475150.12182264105383776No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGG462480.11857420821757104No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGA452130.11592059496931847No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT449640.11528218946321711No Hit
GGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGG443400.11368232988166191No Hit
CAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG442930.11356182763753839No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTC440050.11282343090758983No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGC436780.11198504295379408No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT420630.1078443807355062No Hit
GTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCA415110.10642912033643817No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT414160.10618555197065653No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATA405470.10395754239313816No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACT402940.10330888137689864No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT401860.10303198260316793No Hit
CTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCATG394280.1010885634319839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)