FastQCFastQC Report
Tue 4 May 2021
TC4_A5_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_A5_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35426202
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCA648360.18301707871478856No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTC563020.15892756440557756No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATC520780.14700418633642975No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA513910.14506494373853568No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC497440.14041584248856256No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT476660.13455012761458313No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC476560.13452189992029065No Hit
GGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATC438540.12378973055028591No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATG435640.12297112741580371No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT432650.12212711935645824No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA421720.11904183237028908Illumina Single End PCR Primer 1 (100% over 52bp)
GTTGCAAGGCTGCTGACAAGGACACCTGCTTCTCGACTGAGGGTCCAAACCT401830.11342734397551282No Hit
AGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAA377670.10660753303444721No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTC377550.10657365980129624No Hit
GGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCC369980.10443682334335475No Hit
CCTAAACACATCACAACCACAACCTTCTCAGGCTACCCTGAGAAAAAAAGAC366540.10346579065969308No Hit
GCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGC360490.10175801515499742No Hit
AGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTG360400.10173261023013419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)