FastQCFastQC Report
Tue 4 May 2021
TC4_B3_mDEHP_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_B3_mDEHP_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35414571
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTC614580.1735387391816775No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCA611570.17268880653672186No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTC581090.16408217962036023No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCA545200.15394793290027428No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTAT543410.15344249122769268No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC537620.15180757095716338No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATG518510.14641148695546813No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG508770.14366120656946543No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATC482990.13638171700569238No Hit
AGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTG445580.12581826841838634No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT433080.1222886477997997No Hit
CCACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTGAT419550.11846818644224152No Hit
AGGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATAT396110.11184944185826788No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA384640.1086106619786528No Hit
CTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCC372810.10527022902522241No Hit
GGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCC369680.10438641202232832No Hit
CACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTGATC359110.10140176482725148No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT355830.10047559237693433No Hit
CAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCC354410.10007462747466289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)