FastQCFastQC Report
Tue 4 May 2021
TC4_B4_mDEHP_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_B4_mDEHP_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35427668
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCC33520359.46163038447803TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATGCC9394042.651611164471791TruSeq Adapter, Index 13 (97% over 39bp)
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT852620.24066500792544404No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGC681960.1924936182646851TruSeq Adapter, Index 13 (97% over 40bp)
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA669060.188852396381269No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG617750.17436936577366594No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA569530.16075853482650906No Hit
TGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT553910.15634955142969048No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC529040.14932961435677902No Hit
CTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCATG496560.14016163863791428No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT462720.13060978216234836No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT444560.12548384499933782No Hit
TGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTGTG416490.11756065908712932No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC387730.10944271014394738No Hit
GCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGG372380.10510993836794452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)