FastQCFastQC Report
Tue 4 May 2021
TC4_B7_mDEHP_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_B7_mDEHP_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31153982
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT2631800.8447716250205191TruSeq Adapter, Index 6 (100% over 52bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG1726140.5540672136229648TruSeq Adapter, Index 6 (100% over 52bp)
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA692170.22217705588967726No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT573060.18394438309683814No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCG573030.18393475350919827No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG557910.1790814413387027No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAG459890.1476183686566937No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCC459030.14734232047768403No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGAC444050.1425339463828412No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC411950.13223028760817798No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT406300.1304167152693354No Hit
GTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAAATCTCATTCAGGG352850.11325999995762982No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT335380.10765237008867759No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATG325150.10436868070348118No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGG318810.10233362784892153No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC316160.10148301427406616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)