FastQCFastQC Report
Tue 4 May 2021
TC4_B7_mDEHP_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_B7_mDEHP_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31153982
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT2871270.9216382034245253Illumina Single End PCR Primer 1 (100% over 52bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA1789260.5743278660172558Illumina Single End PCR Primer 1 (100% over 52bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG1551530.4980198036963621No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA764070.24525596759990423No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGT373340.1198370083156625No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA323330.1037844857199956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)