FastQCFastQC Report
Tue 4 May 2021
TC4_C3_mPb_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_C3_mPb_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43647475
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA1471650.337167270271648No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC1365760.3129069894650263No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1246350.2855491640696283No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT1150220.2635249805401114No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG1099490.25190231508237304No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT747370.17122869077764521No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC675740.15481766127364754No Hit
GGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCT636380.14579995750040523No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT623320.1428078027423121No Hit
GGTGTATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCT573460.13138446152956157No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGA564650.12936601716365037No Hit
GGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGG562520.12887801642592153No Hit
GTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAAATCTCATTCAGGG551060.126252434991944No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG548190.12559489409181174No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATAT539240.12354437456004043No Hit
GCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGG518430.11877663026326264No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG503570.11537208051553954No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCC500500.11466871794989288No Hit
AGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGA496800.11382101713787567No Hit
GCCTTGTCTAAATTCTCTTGCTGACTCTCTTGTAGACAATGTCGCCCAATGT496220.11368813430788378No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATG492750.1128931284112082No Hit
GGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACA490290.11232952192538057No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATA466230.1068171755639931No Hit
GGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCT460610.10552958676303727No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGAC457440.10480331336463335No Hit
GGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGG449990.10309645632422036No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACT446910.10239080267529795No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGG442430.10136439736777442No Hit
GATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTC441960.10125671645381548No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAG440210.10085577688056412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)