FastQCFastQC Report
Tue 4 May 2021
TC4_C3_mPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_C3_mPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43647475
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG1290930.2957628133127976No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATC1049960.2405545796177213No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA978580.2242008271956167No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCA889660.20382851470789548No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTC866420.19850403717511725No Hit
GGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATC764420.17513498776275147No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATG672180.15400203562749049No Hit
GCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGC655380.15015301572427728No Hit
GGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCC635920.14569456766972202No Hit
AGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAA620680.14220295675752148No Hit
AGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTG591690.13556110634120302No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA578090.1324452330862209No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCA571180.1308620945426969No Hit
AGGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATAT565600.12958367007484395No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTC555560.12728342246601893No Hit
GGAAAGGTTTGCACAACTATGTGAGAAGCATGGAATCCTTAGAGAAAATATC523600.11996112031681098No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTAT500350.11463435170075705No Hit
GTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCA500000.11455416378610675No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT492410.11281523157983366No Hit
GAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTCC491230.11254488375329844No Hit
CTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATCCCCAG472450.10824222936149228No Hit
GCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTG449850.10306438115836025No Hit
GTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGAC447400.10250306575580832No Hit
CACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTGATC439070.10059459338713178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)