FastQCFastQC Report
Tue 4 May 2021
TC4_C4_mPb_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_C4_mPb_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49625808
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1136490.2290118883303623No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG994260.20035139780494857No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG927120.1868221470570313No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCG658120.13261648052158667No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC618120.12455615836018227No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT602910.12149122085830825No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA585620.1180071466040412No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGAC584090.11769883928136747No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT583700.11762025114029377No Hit
GATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTC546730.11017049838261575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)