FastQCFastQC Report
Tue 4 May 2021
TC4_C5_mPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_C5_mPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41231588
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC635410.15410757402795158No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA620670.1505326450196388Illumina Single End PCR Primer 1 (100% over 52bp)
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA585560.14201732904393594No Hit
GTTGCAAGGCTGCTGACAAGGACACCTGCTTCTCGACTGAGGGTCCAAACCT529030.12830696697881247No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG486630.11802358909872691No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC467180.11330633202873486No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT466280.11308805278128023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)