FastQCFastQC Report
Tue 4 May 2021
TC4_D6_fCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_D6_fCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66218846
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA2910320.4395002594880617No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT2454040.37059540421468534No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG2349110.35474946210932157No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT1747350.26387503038032406No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT1201310.18141512161054574No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG1022220.15436995081430444No Hit
CTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCATG1010300.15256985903982684No Hit
CACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGGTGTT919830.13890758531189143No Hit
GTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCC894570.13509296129986922No Hit
GCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGG867670.13103067365444573No Hit
CCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCAGCCTTGCAACATGTATCCAG856090.1292819267795757No Hit
GTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAAATCTCATTCAGGG827750.12500217838287306No Hit
GTGGTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTT819920.12381973554779255No Hit
GTCCTTGTCAGCAGCCTTGCAACATGTATCCAGGAACTGTGCAAAGTCATCC799950.1208039777679001No Hit
ATTTTTTATTAATTGCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATT763080.11523607644868955No Hit
GTCTTCAGTTGCTCCGCTGTAGCCTTGGGCTTGTGCTTCACCAGCTCAGCAA753490.11378784825093449No Hit
GTGTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTC725500.10956095489794553No Hit
CCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGCTGTAGC695520.10503354286784158No Hit
GATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTC693370.10470886188502893No Hit
GGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCGAGAAGCAGGTG664680.10037625844461259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)