FastQCFastQC Report
Tue 4 May 2021
TC4_F1_fPb_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F1_fPb_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32646368
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCC2632670.8064204875715424TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGC1790660.548502056951634TruSeq Adapter, Index 15 (97% over 40bp)
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1079700.33072591719850736No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT832280.25493800719271437No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG794390.2433318156555731No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT602950.18469129552175606No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTATGCC435620.1334359767065053TruSeq Adapter, Index 15 (97% over 39bp)
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT417080.12775693761707274No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG409480.12542896042830862No Hit
GTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAAATCTCATTCAGGG362400.11100775436949065No Hit
GTCTTCAGTTGCTCCGCTGTAGCCTTGGGCTTGTGCTTCACCAGCTCAGCAA348190.10665504965207769No Hit
GTCCTTGTCAGCAGCCTTGCAACATGTATCCAGGAACTGTGCAAAGTCATCC338870.10380021446796164No Hit
CCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCAGCCTTGCAACATGTATCCAG338350.1036409318182041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)