FastQCFastQC Report
Tue 4 May 2021
TC4_F2_fPb_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F2_fPb_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30832563
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG5568551.8060613384621966TruSeq Adapter, Index 12 (100% over 52bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT1771620.5745938149870966TruSeq Adapter, Index 12 (100% over 52bp)
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG1056610.3426928860892946No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1024460.33226559854917026No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT856970.27794316028803706No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG663650.215243215427793No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT632870.20526026331317315No Hit
GTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAAATCTCATTCAGGG464160.15054213949064174No Hit
GATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTC453490.1470815124905445No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT398380.12920755241787718No Hit
CCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCAGCCTTGCAACATGTATCCAG384590.12473500824436813No Hit
GCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGG377950.1225814409265944No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA373160.12102788859946544No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC361500.11724617249626637No Hit
GTCCTTGTCAGCAGCCTTGCAACATGTATCCAGGAACTGTGCAAAGTCATCC347880.11282876483541118No Hit
GTCTTCAGTTGCTCCGCTGTAGCCTTGGGCTTGTGCTTCACCAGCTCAGCAA315300.1022620143515153No Hit
GTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCC309010.10022196338332302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)