FastQCFastQC Report
Tue 4 May 2021
TC4_F2_fPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F2_fPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30832563
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA5789341.8776706951024476Illumina Single End PCR Primer 1 (100% over 52bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT1940730.6294416717805782Illumina Single End PCR Primer 1 (100% over 52bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG619770.2010115085145533No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC429480.13929429090925718No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC381240.12364849461265999No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT347660.11275741170138856No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA346830.11248821578666686No Hit
GTTGCAAGGCTGCTGACAAGGACACCTGCTTCTCGACTGAGGGTCCAAACCT331660.10756809286337954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)