FastQCFastQC Report
Tue 4 May 2021
TC4_F4_fPb_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F4_fPb_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37343735
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1034550.2770344209008553No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG922700.24708294443499024No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG778320.20842050212706362No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT611570.163767764525964No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT564870.1512623201723127No Hit
GATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTC515920.1381543651163977No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC441340.11818314370536318No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA432440.11579987914974224No Hit
GGTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGG414590.11101996091178346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)