FastQCFastQC Report
Wed 3 Mar 2021
TC3_A1_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A1_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50168211
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC1727860.344413317827897No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1596340.318197513560928No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1510580.3011030231873327No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT1253720.24990327041958899No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA1157880.2307995395729778No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA1153520.2299304633366336No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC1130950.22543159850766853No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT1129230.225088751919019No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC1102890.219838415206793No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG1093230.21791289308681944No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG1042690.20783878460405933No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC1012000.2017213649496092No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT998350.19900051847573358No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT993320.19799789153334568No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA973900.19412691435219806No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT896570.17871277092180943No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA895520.1785034750392036No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC889570.17731746503777063No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT818680.1631870030206977No Hit
CACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATT761470.15178336735986062No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT706070.14074051793475353No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA698580.13924754063883205No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTC686670.13687352734184602No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA681850.1359127595759793No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA634090.12639278685859456No Hit
CCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGG632770.12612967203474726No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTT629780.12553367709285068No Hit
GGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGT626530.12488585650383267No Hit
CCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTG624400.12446128485626086No Hit
CAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATA601830.11996242002729578No Hit
CCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAAT583960.11640040343475673No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG579350.11548149484541118No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCCGAGCACTCTTC555750.11077732072208037No Hit
ATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGG551390.1099082444857362No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG545930.10881990589618593No Hit
CACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCT544780.10859067707237956No Hit
CTTCATTCTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGAT538400.10731895542378421No Hit
GGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGA536600.10696016248217421No Hit
CTCTTTTGTCAGAGGCCAGGATAATAGTATGCCATTCCCCATTAATCTTT533380.10631832177551638No Hit
ATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCA507040.10106798506329037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)