FastQCFastQC Report
Wed 3 Mar 2021
TC3_A1_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A1_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50168211
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT2894220.5769031708146818No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC2726070.5433859301859498No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1994970.39765619706869754No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1732440.3453262465348824No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC1696180.33809856205556144No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA1656280.3301453185165403No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1649660.3288257578090636No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA1295440.25821929348846023No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT1233420.2458568833558765No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA880500.17550954727088036No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC826990.16484343043446376No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA794640.15839512395608446No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC716280.14277567123133014No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAA683790.13629945863527004No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG660890.13173481510034313No Hit
CATTAACGAAAAGGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCC659450.13144778074705515No Hit
CCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTAT646900.12894619662638557No Hit
CAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATC626430.12486592356263213No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGAA603630.12032121296890574No Hit
GATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCT589000.11740502367126465No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCT578460.11530409166872625No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT575620.1147379961386305No Hit
CTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAGACTTTTTCTG556120.11085107260452243No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC554180.11046437354523167No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATA552020.1100338220152997No Hit
GCTAGTTCTACGGGAAGGAACTTTAATGTAGAAAAGATTAATGGGGAATG547500.10913285307303464No Hit
CTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGC533900.10642197306975926No Hit
GACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGTATGATGGATTCAA532180.10607912648110973No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCAC523330.10431506118486068No Hit
CTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGACTAT504890.10063942682747846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)