FastQCFastQC Report
Wed 3 Mar 2021
TC3_A2_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A2_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59522736
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1233880.20729557861721948No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC1178160.1979344497873888No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1151920.19352605028102204No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC906730.15233338736310775No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC878610.1476091421604007No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA850210.1428378561093025No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC826630.13887634466265125No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG810630.1361882961831593No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT803870.13505259570057399No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA802640.13484595197371302No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT800210.1344377046108902No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA760660.12779318477564608No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG741800.12462464763044494No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA727990.12230452578658346No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT719240.12083449927436131No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG700090.1176172412504694No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC696170.11695866937299387No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT681250.11445206416586765No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT659960.11087527965784369No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG655850.11018478720467419No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT607950.1021374420691952No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT599640.10074133689015909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)