FastQCFastQC Report
Wed 3 Mar 2021
TC3_A3_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A3_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42673190
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1637390.3837046164113815No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC1320660.3094823705469406No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG1112890.26079372083502544No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA1079790.25303709425051185No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA937200.2196226717524516No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG794540.18619184551236972No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC731720.1714706587438155No Hit
AGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGG670950.1572298672773233No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA644610.1510573734937557No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG615890.14432715248145264No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA515370.120771378938392No Hit
GTTGCAAGTCAGCTAAATACTTTGACACCGGTAGGAATTGCGATAATTAT510230.11956687559566087No Hit
GGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGT508880.11925051771381516No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT475300.11138140832686752No Hit
GGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTCCA475180.11135328762625903No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTT475010.11131344996706362No Hit
GTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAAT465070.10898411859999217No Hit
GGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCA460900.10800692425384649No Hit
GTTTAGGTTGCGGTCTGTTAGTAGTATAGTAATGCCTGCGGCTAGCACTG446520.10463712696426022No Hit
GTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTC443190.10385677752237411No Hit
GGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATC442660.10373257776135321No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA433190.1015133858049984No Hit
GGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACA427400.10015656200063787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)