FastQCFastQC Report
Wed 3 Mar 2021
TC3_A3_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A3_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42673190
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8540892.0014650885017033No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC1299230.3044604820966045No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT800260.18753226557470862No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT749570.17565361295933113No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATA604400.14163459539818796No Hit
GCTAGTTCTACGGGAAGGAACTTTAATGTAGAAAAGATTAATGGGGAATG579320.13575736897100968No Hit
GTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTA499640.11708522376696001No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG487540.11424971978893539No Hit
GTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGGCT477500.11189695450469017No Hit
GGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAG438360.10272491932288165No Hit
GGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCT437630.10255385172751323No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT427070.10007923007396446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)