FastQCFastQC Report
Wed 3 Mar 2021
TC3_A4_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A4_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58978467
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1780370.30186779863233815No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1737580.2946126083609464No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC1558630.2642710262374232No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC1301710.22070936499587213No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT1289600.2186560732411034No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT1245440.21116859480257427No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT1239330.2101326234878231No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA1238280.20995459241082004No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG1237310.20979012560635052No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC1088830.1846148357840498No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC1071020.1815950896112644No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA1046240.17739355619399194No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG1035840.1756302007646282No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT1019470.17285461149744705No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT981790.16646583913413687No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA973680.1650907610060465No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA965750.16374620249115665No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT938060.1590512686604757No Hit
CACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATT924720.15678942621550335No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT886180.15025483792245736No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA812060.13768753942010734No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC806570.1367566912174913No Hit
CCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTG793390.13452197731758608No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA762350.12925903957456203No Hit
CTCCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTG744160.12617486310724219No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTC727400.12333314801145985No Hit
CTTCATTCTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGAT694240.11771075704629624No Hit
GGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGT681060.11547604314639102No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA666750.11304973389694921No Hit
CCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAAT661950.11223587754493518No Hit
CTCGGCCATAGAGCCCCATCAGCTGGAAGGTTTCCCCATCCTTTTCGTTA659550.11182894936892815No Hit
CCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGG658070.11157801032705716No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG653350.1107777182475767No Hit
CTCTTTTGTCAGAGGCCAGGATAATAGTATGCCATTCCCCATTAATCTTT652250.11059120950024015No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTT645500.1094467240052204No Hit
ATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCA639470.10842431696300278No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATTGGC634750.10762402488352232No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG634070.10750872856698698No Hit
ATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGG624550.10589458013549251No Hit
CTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAG623390.10569789818375577No Hit
CAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATA610670.10354117885091858No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG604280.1024577325822999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)