FastQCFastQC Report
Wed 3 Mar 2021
TC3_A4_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A4_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58978467
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC3355000.5688516793764747No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT3261890.5530645616814693No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT2394370.40597359032746644No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC2071500.35122988191605586No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA2059130.3491325062755531No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1893890.32111550135747No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC1869060.3169054902698641No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA1438340.24387544694913824No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT1395430.2365999102689461No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA1081170.18331605668896075No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC964110.16346813490421852No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC875600.14846096287989308No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA810460.137416253969436No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAA773120.13108512976439352No Hit
CATTAACGAAAAGGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCC753480.12775510085740277No Hit
CAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATC742180.12583914736203639No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG730500.12385876357213557No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCT692650.11744116712969158No Hit
CCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTAT664380.11264789232314228No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGAA660770.11203580452506506No Hit
CTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAGACTTTTTCTG653950.11087945029157845No Hit
GATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCT652580.1106471621244411No Hit
GACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGTATGATGGATTCAA645370.10942468206235337No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC639630.10845144550806993No Hit
TGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGA633570.1074239518636522No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCAC619980.10511972106701248No Hit
CTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGACTAT617660.10472635716353902No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATA610850.10357169846411912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)