FastQCFastQC Report
Wed 3 Mar 2021
TC3_A5_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A5_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61059000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT2001650.32782227026318805No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC1927930.3157487020750422No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1427630.23381155931148562No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC1341130.21964493358882392No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA1340150.21948443308930707No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT1329990.217820468726969No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT1280680.20974467318495227No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC1201630.19679817881065853No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC1156590.18942170687367954No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG1153820.18896804729851455No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA1106650.18124273243911626No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT1079390.1767781981362289No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT1066600.17468350284151393No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT1024400.16777215480109403No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG1017980.1667207127532387No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC959250.15710214710362108No Hit
CACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATT883670.14472395551843298No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT853450.13977464419659674No Hit
CCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGG849880.1391899638054996No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA827450.1355164676788025No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTC818850.13410799390753206No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT799900.1310044383301397No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA790990.1295451939926956No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA771420.12634009728295584No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG763370.12502170032263876No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA745360.12207209420396666No Hit
CAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATA717510.11751093204932933No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA713420.11684108812787632No Hit
ATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGG684190.11205391506575607No Hit
CCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTG675080.11056191552432894No Hit
CAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG648830.10626279500155586No Hit
CCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAAT644490.10555200707512408No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTT629460.10309045349579915No Hit
CTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGA622050.10187687318822779No Hit
CTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAG612490.1003111744378388No Hit
CTTCATTCTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGAT612350.10028824579505068No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG611450.10014084737712703No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCG611230.1001048166527457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)