FastQCFastQC Report
Wed 3 Mar 2021
TC3_A6_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A6_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56941437
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1487190.2611788669822295No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC1273910.2237228400119231No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1146570.20135951258132107No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC1016730.1785571375727662No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT923590.16219998100855795No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT882520.15498730739794994No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG875110.15368597037689793No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC859950.15102358586419234No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA858780.15081811159771047No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA856840.15047741067721912No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC849390.1491690488949199No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT747510.13127698199818877No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG746190.13104516487702972No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT739520.12987378593905174No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT697910.12256627805160589No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT697110.12242578282666101No Hit
CACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATT679010.119247078362283No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT673740.11832156606795856No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC671390.11790886134468295No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG669320.11754532995013807No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA644930.11326198177963089No Hit
CTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGC625290.10981282400723397No Hit
CCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTG613400.10772471372649059No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG604310.10612833673305434No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTC601370.1056120167813819No Hit
CCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGG597060.10485509875699132No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA593360.10420530834162123No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA591030.10379611599896925No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA573370.10069468390831093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)